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DNP-NMR Literature Blog

Get up-to-date articles about dynamic nuclear polarization enhanced NMR spectroscopy
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School-Conference “Spinus”

Dear colleagues,
Please, help us to disseminate information:

Welcome to the 18th International School-Conference “Spinus-2021. Magnetic Resonance and its Applications” organized by the Saint
Petersburg State University from March 29 to April 2, 2021. This time
the conference will be held online format (via ZOOM) due to
limitations of the COVID-19 epidemic. The goal of “Spinus” is to
provide to young scientists a platform for discussion of the use of
all aspects of magnetic resonance methods and techniques, as well as
computational and theoretical approaches, for the solving of
fundamental and applied problems in physics, chemistry, medicine and biology. The official language of the “Spinus” is English.
The scope of the “Spinus” Conference includes the following topics:
  Modern trends in NMR, ESR and NQR
  Magnetic resonance for fundamental science
  Magnetic resonance imaging
  Computer Modeling
  NMR in the Earth magnetic field
  Magnetic resonance for industry
  Related areas
Extended abstracts will be published in the Book of Proceedings.
Selected papers of participants will be published in a special issue
of the journal “Applied Magnetic Resonance”.

More information and applications for “Spinus 2021” are available on the website

Vladimir Chizhik
Scientific adviser of the School-Conference “Spinus”

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NMR web database:

Diffusion measurements with continuous hydrogenation in PHIP

Bussandri, S., L. Buljubasich, and R.H. Acosta. “Diffusion Measurements with Continuous Hydrogenation in PHIP.” Journal of Magnetic Resonance 320 (November 2020): 106833.

DOSY is a powerful spectroscopic NMR technique that resolves components in mixtures through the evaluation of different diffusion coefficients. The application of DOSY to dilute mixtures is hampered by the low signal to noise ratios (SNR), leading to long acquisition times. The use of PHIP may resolve this issue as long as reproducible signals are obtained in order to perform 2D experiments. Here we show that the use of hollow membranes and adequate gas flow produce constant polarization for a time-span that enables the acquisition of 2D experiments. A pressure gradient is evidenced by the presence of convection, which is accounted for by using a DPGSE sequence. The influence of J-coupling evolution during the sequence is studied both numerically and experimentally, to determine the optimum echo-time. The applicability of the method for samples with poor SNR is explored by setting the reaction rate to achieve a low intensity of polarized signals.

SQUID-based ultralow-field MRI of a hyperpolarized material using signal amplification by reversible exchange

Lee, Seong-Joo, Keunhong Jeong, Jeong Hyun Shim, Hyun Joon Lee, Sein Min, Heelim Chae, Sung Keon Namgoong, and Kiwoong Kim. “SQUID-Based Ultralow-Field MRI of a Hyperpolarized Material Using Signal Amplification by Reversible Exchange.” Scientific Reports 9, no. 1 (December 2019): 12422.

The signal amplification by reversible exchange (SABRE) technique is a very promising method for increasing magnetic resonance (MR) signals. SABRE can play a particularly large role in studies with a low or ultralow magnetic field because they suffer from a low signal-to-noise ratio. In this work, we conducted real-time superconducting quantum interference device (SQUID)-based nuclear magnetic resonance (NMR)/magnetic resonance imaging (MRI) studies in a microtesla-range magnetic field using the SABRE technique after designing a bubble-separated phantom. A maximum enhancement of 2658 for 1H was obtained for pyridine in the SABRE-NMR experiment. A clear SABRE-enhanced MR image of the bubble-separated phantom, in which the para-hydrogen gas was bubbling at only the margin, was successfully obtained at 34.3 μT. The results show that SABRE can be successfully incorporated into an ultralow-field MRI system, which enables new SQUID-based MRI applications. SABRE can shorten the MRI operation time by more than 6 orders of magnitude and establish a firm basis for future low-field MRI applications.

[NMR] Postdoctoral position – DNP – Debelouchina lab – UCSD

Postdoctoral position – Debelouchina lab

UCSDSensitivity-enhanced NMR spectroscopy in cells

The Debelouchina lab at UCSD has an NIH-funded position for a postdoctoral researcher to work on the development of in-cell DNP methodologies and applications. Candidates should have expertise in biomolecular solid-state NMR spectroscopy or solid-state NMR/DNP methodology development, and interest in learning chemical biology and cell biology techniques. The project will be supported by a dedicated 600 MHz DNP NMR spectrometer equipped with 1.9 mm and 3.2 mm low temperature probes. In addition, UCSD has outstanding resources for magnetic resonance spectroscopy including 900 MHz, 750 MHz, and 2 x 700 MHz solid-state NMR spectrometers; 800 MHz, 600 MHz and 500 MHz solution NMR spectrometers; and an X-band EPR spectrometer. UCSD and the San Diego area offer numerous opportunities for networking and collaboration with other NMR spectroscopy, cryo-EM, cryo-ET, imaging, synthetic chemistry and biology groups. Candidates should send a cover letter, CV and the names of three references to gdebelouchina@UCSD.EDU
For more information, take a look at our website and publications:

Galia Debelouchina, Ph.D.
Assistant Professor
Department of Chemistry and Biochemistry
University of California, San Diego
9500 Gilman Drive
La Jolla, CA 92093

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Scalable dissolution-dynamic nuclear polarization with rapid transfer of a polarized solid #DNPNMR

Kouřil, Karel, Hana Kouřilová, Samuel Bartram, Malcolm H. Levitt, and Benno Meier. “Scalable Dissolution-Dynamic Nuclear Polarization with Rapid Transfer of a Polarized Solid.” Nature Communications 10, no. 1 (December 2019): 1733.

In dissolution-dynamic nuclear polarization, nuclear spins are hyperpolarized at cryogenic temperatures using radicals and microwave irradiation. The hyperpolarized solid is dissolved with hot solvent and the solution is transferred to a secondary magnet where strongly enhanced magnetic resonance signals are observed. Here we present a method for transferring the hyperpolarized solid. A bullet containing the frozen, hyperpolarized sample is ejected using pressurized helium gas, and shot into a receiving structure in the secondary magnet, where the bullet is retained and the polarized solid is dissolved rapidly. The transfer takes approximately 70 ms. A solenoid, wound along the entire transfer path ensures adiabatic transfer and limits radical-induced low-field relaxation. The method is fast and scalable towards small volumes suitable for high-resolution nuclear magnetic resonance spectroscopy while maintaining high concentrations of the target molecule. Polarization levels of approximately 30% have been observed for 1-13C-labelled pyruvic acid in solution.

Lignin-polysaccharide interactions in plant secondary cell walls revealed by solid-state NMR #DNPNMR

Kang, Xue, Alex Kirui, Malitha C. Dickwella Widanage, Frederic Mentink-Vigier, Daniel J. Cosgrove, and Tuo Wang. “Lignin-Polysaccharide Interactions in Plant Secondary Cell Walls Revealed by Solid-State NMR.” Nature Communications 10, no. 1 (December 2019): 347.

Lignin is a complex aromatic biopolymer that strengthens and waterproofs plant secondary cell walls, enabling mechanical stability in trees and long-distance water transport in xylem. Lignin removal is a key step in paper production and biomass conversion to biofuels, motivating efforts to re-engineer lignin biosynthesis. However, the physical nature of lignin’s interactions with wall polysaccharides is not well understood. Here we show that lignin selfaggregates to form highly hydrophobic and dynamically unique nanodomains, with extensive surface contacts to xylan. Solid-state NMR spectroscopy of intact maize stems, supported by dynamic nuclear polarization, reveals that lignin has abundant electrostatic interactions with the polar motifs of xylan. Lignin preferentially binds xylans with 3-fold or distorted 2-fold helical screw conformations, indicative of xylans not closely associated with cellulose. These findings advance our knowledge of the molecular-level organization of lignocellulosic biomass, providing the structural foundation for optimization of post-harvest processing for biofuels and biomaterials.

[NMR] Postdoctoral position @NHMFL

A postdoctoral position is available from Feburary 2021 for solid-state NMR at the National High Magnetic Field Laboratory (NHMFL) in Tallahassee, Florida. This postdoc will support the operation and development of the 36T/1.5GHz Series-Connected-Hybrid (SCH) NMR program for material and biological applications. The NHMFL has a wide array of state-of-art NMR instruments from 600 MHz to 1.5 GHz including the 36 T SCH magnet and a 600 MHz MAS DNP system. The NMR/MRI program has an active and stimulating research and development environment with a number of staff scientists and engineers. This postdoc will have opportunities and support for independent and collaborative research.

Minimum qualifications include a Ph.D. in Chemistry, Physics, Biology or a related discipline. To apply, please send a CV, a cover letter describing your experience and research interests, and contact information for three references to

Zhehong Gan ( or Robert Schruko (
National High Magnetic Field Laboratory
1800 E. Paul Dirac Dr., Tallahassee, FL 32310, USA

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Nuclear spin-hyperpolarization generated in a flavoprotein under illumination: experimental field-dependence and theoretical level crossing analysis

Ding, Yonghong, Alexey S. Kiryutin, Alexandra V. Yurkovskaya, Denis V. Sosnovsky, Renad Z. Sagdeev, Saskia Bannister, Tilman Kottke, et al. “Nuclear Spin-Hyperpolarization Generated in a Flavoprotein under Illumination: Experimental Field-Dependence and Theoretical Level Crossing Analysis.” Scientific Reports 9, no. 1 (December 2019): 18436.

The solid-state photo-chemically induced dynamic nuclear polarization (photo-CIDNP) effect generates non-equilibrium nuclear spin polarization in frozen electron-transfer proteins upon illumination and radical-pair formation. The effect can be observed in various natural photosynthetic reaction center proteins using magic-angle spinning (MAS) nuclear magnetic resonance (NMR) spectroscopy, and in a flavin-binding light-oxygen-voltage (LOV) domain of the blue-light receptor phototropin. In the latter system, a functionally instrumental cysteine has been mutated to interrupt the natural cysteineinvolving photochemistry allowing for an electron transfer from a more distant tryptophan to the excited flavin mononucleotide chromophore. We explored the solid-state photo-CIDNP effect and its mechanisms in phototropin-LOV1-C57S from the green alga Chlamydomonas reinhardtii by using fieldcycling solution NMR. We observed the 13C and, to our knowledge, for the first time, 15N photo-CIDNP signals from phototropin-LOV1-C57S. Additionally, the 1H photo-CIDNP signals of residual water in the deuterated buffer of the protein were detected. The relative strengths of the photo-CIDNP effect from the three types of nuclei, 1H, 13C and 15N were measured in dependence of the magnetic field, showing their maximum polarizations at different magnetic fields. Theoretical level crossing analysis demonstrates that anisotropic mechanisms play the dominant role at high magnetic fields.

Glyoxalase activity in human erythrocytes and mouse lymphoma, liver and brain probed with hyperpolarized 13C-methylglyoxal #DNPNMR #dDNP

Shishmarev, Dmitry, Philip W. Kuchel, Guilhem Pagès, Alan J. Wright, Richard L. Hesketh, Felix Kreis, and Kevin M. Brindle. “Glyoxalase Activity in Human Erythrocytes and Mouse Lymphoma, Liver and Brain Probed with Hyperpolarized 13C-Methylglyoxal.” Communications Biology 1, no. 1 (December 2018): 232.

Methylglyoxal is a faulty metabolite. It is a ubiquitous by-product of glucose and amino acid metabolism that spontaneously reacts with proximal amino groups in proteins and nucleic acids, leading to impairment of their function. The glyoxalase pathway evolved early in phylogeny to bring about rapid catabolism of methylglyoxal, and an understanding of the role of methylglyoxal and the glyoxalases in many diseases is beginning to emerge. Metabolic processing of methylglyoxal is very rapid in vivo and thus notoriously difficult to detect and quantify. Here we show that 13C nuclei in labeled methylglyoxal can be hyperpolarized using dynamic nuclear polarization, providing 13C nuclear magnetic resonance signal enhancements in the solution state close to 5,000-fold. We demonstrate the applications of this probe of metabolism for kinetic characterization of the glyoxalase system in isolated cells as well as mouse brain, liver and lymphoma in vivo.

[NMR] Solid-state NMR specialist at EPFL

The Institute of Chemistry and Chemical Engineering at EPFL (ISIC; is currently looking for a full-time specialist in solid-state NMR for its NMR platform ( 

As solid-state NMR specialist, you will have the responsibility to advise and guide EPFL researchers wishing to characterize their samples by solid-state NMR. A major part of the work will be dedicated to the preparation and measurement of these samples.

You will be in charge of the solid-state spectrometers of the ISIC NMR platform (two 400 MHz routine spectrometers, and four research spectrometers at 400, 500 and 900 MHz able to work at low temperature (100 K), fast MAS (100 kHz) with possible coupling to DNP. 

The user pool is large and diverse, (chemistry, material sciences, life sciences, physics …), and we are thus looking for an open-minded, well organized and flexible person with a modern vision and knowledge of solid state NMR and service, who will integrate smoothly into our existing team.
Candidates should submit their application online before 15.01.2021.

More details on the position and the application process here: :
For additional information, please contact Dr Aurélien Bornet (NMR platform leader, or Prof. Sandrine Gerber (Responsible for ISIC Platforms,
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