Sadet, Aude, Cristina Stavarache, Mihaela Bacalum, Mihai Radu, Geoffrey Bodenhausen, Dennis Kurzbach, and Paul R. Vasos. “Hyperpolarized Water Enhances Two-Dimensional Proton NMR Correlations: A New Approach for Molecular Interactions.” Journal of the American Chemical Society 141, no. 32 (August 14, 2019): 12448–52.
Proteins and peptides interactions are characterized in the liquid state by multidimensional NMR spectroscopy experiments, which can take hours to record. We show that, starting from hyperpolarized HDO, two-dimensional (2D) proton correlation maps of a peptide, either free in solution or interacting with liposomes, can be acquired in less than 60 s. In standard 2D NMR spectroscopy without hyperpolarization, the acquisition time required for similar spectral correlations is of the order of hours. This hyperpolarized experiment allows to identify amino-acids featuring solvent-interacting hydrogens and provides fast spectroscopic analysis of peptide conformers. These experiments are a useful and straightforward tool for biochemistry and structural biology, as they do not recur to nitrogen-15 or carbon-13 isotope enrichment.