Effective PHIP labeling of bioactive peptides boosts the intensity of the NMR signal

Sauer, G., et al., Effective PHIP labeling of bioactive peptides boosts the intensity of the NMR signal. Angew Chem Int Ed Engl, 2014. 53(47): p. 12941-5.

http://www.ncbi.nlm.nih.gov/pubmed/25296746

A series of novel bioactive derivatives of the sunflower trypsin inhibitor-1 (SFTI-1) suitable for hyperpolarization by parahydrogen-induced polarization (PHIP) was developed. The PHIP activity was achieved by labeling with L-propargylglycine, O-propargyl-L-tyrosine, or 4-pentynoic acid. (1) H NMR signal enhancements (SE) of up to a factor of 70 were achieved in aqueous solution. We found that an isolated spatial location of the triple bond within the respective label and its accessibility for the hydrogenation catalyst are essential factors for the degree of signal enhancement.

Might this article interest your colleagues? Share it!

Have a question?

If you have questions about our instrumentation or how we can help you, please contact us.