Category Archives: EPR

[NMR] Postdoctoral position at Miami University

Postdoctoral Position: EPR Spectroscopic Studies of Membrane Proteins

Miami University, Oxford, OH, USA

A Postdoctoral research position is available immediately to study the structural and dynamic properties of integral membrane proteins in the laboratory of Prof. Gary A. Lorigan in the Department of Chemistry and Biochemistry at Miami University in Ohio. The postdoctoral position is funded through a NIH MIRA R35 grant.

Candidates who are interested in studying the structural and dynamic properties of membrane proteins are encouraged to apply. Experience in two of the following areas is desirable: molecular biology and biochemistry of membrane proteins, protein purification, and EPR spectroscopy. 2 pulsed EPR spectrometers (X-band/Q-band) for DEER and ESEEM experiments, 2 CW-EPR spectrometers, and a 500 MHz solid-state NMR instrument are available for this project. Miami University is home to the Ohio Advanced EPR Lab (http://epr.miamioh.edu). Please send a CV and two letters of recommendation to: Professor Gary A. Lorigan, Department of Chemistry and Biochemistry, Miami University gary.lorigan@miamioh.edu. A Ph.D. in Chemistry/Biochemistry or related fields is required. Contact phone is 513-529-3338. 

Miami University, an Equal Opportunity/Affirmative Action employer, encourages applications from minorities, women, protected veterans and individuals with disabilities. Miami University prohibits harassment, discrimination and retaliation on the basis of sex/gender (including sexual harassment, sexual violence, sexual misconduct, domestic violence, dating violence, or stalking), race, color, religion, national origin (ancestry), disability, age (40 years or older), sexual orientation, gender identity, pregnancy, status as a parent or foster parent, military status, or veteran status in its recruitment, selection, and employment practices. Requests for all reasonable accommodations for disabilities related to employment should be directed to ADAFacultyStaff@miamioh.edu or 513-529-3560.

As part of the University’s commitment to maintaining a healthy and safe living, learning, and working environment, we encourage you to read Miami University’s Annual Security & Fire Safety Report at http://www.MiamiOH.edu/campus-safety/annual-report/index.html(http://www.miamioh.edu/campus-safety/annual-report/index.html), which contains information about campus safety, crime statistics, and our drug and alcohol abuse and prevention program designed to prevent the unlawful possession, use, and distribution of drugs and alcohol on campus and at university events and activities. This report also contains information on programs and policies designed to prevent and address sexual violence, domestic violence, dating violence, and stalking. Each year, email notification of this website is made to all faculty, staff, and enrolled students. Written notification is also provided to prospective students and employees. Hard copies of the Annual Security & Fire Safety Report may be obtained from the Miami University Police Department at (513) 529-2225.

Gary A. Lorigan

John W. Steube Professor 

Department of Chemistry and Biochemistry

Miami University

651 E. High St.

Oxford, Ohio 45056

Office: 137 Hughes Laboratories

Phone: (513) 529-3338

Fax: (513) 529-5715

e-mail: gary.lorigan@miamioh.edu

web: www.users.muohio.edu/lorigag/index.html

EPR facility: epr.muohio.edu

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EasySpin Academy, Aug 26-28 2019, Seattle

Dear EPR community,

We are excited to announce EasySpin Academy 2019, a 2.5-day workshop for EasySpin, a MATLAB-based software toolbox for simulating and fitting Electron Paramagnetic Resonance (EPR) spectra – see http://easyspin.org.

The workshop will be held at the University of Washington in Seattle. It starts on Monday Aug 26 at 6 pm and ends on Wednesday Aug 28 in the evening. Instructors include Stefan Stoll (creator of EasySpin), Stephan Pribitzer (active developer), and others.

The workshop is open to everyone. Some experience with EPR spectroscopy is required, but no prior experience with either MATLAB or EasySpin are expected. We will be able to accommodate both beginners and users with EasySpin experience.

This workshop is a unique opportunity to learn directly from the core developers of EasySpin, to interact with other EPR researchers, to discuss your EPR simulation projects and needs with EasySpin developers, and to provide feedback about desired features for future releases of EasySpin.

For the workshop, bring your own laptop with MATLAB pre-installed. If you do not have access to a licensed copy of MATLAB, you can download a 30-day trial from http://mathworks.com.

The registration fee is $295 and covers three nights in on-campus double-occupancy dorm rooms (Monday, Tuesday, Wednesday) and all meals during the workshop.

To register, please visit http://easyspin.org/academy. After registration, you will received an email with details about the payment. The registration deadline is July 25.

There are only a limited number of slots available, and they will be allocated on a first-come, first-served basis.

Best regards

Stefan Stoll

[NMR] REWIRE (reinforcing Women in research) Program at the University of Vienna #DNPNMR

Dear colleagues,

The NMR lab of the university of Vienna is participating in the REWIRE (Reinforcing Women in Research, https://rewire.univie.ac.at/) program, a Marie Sklodowska Curie Actions COFUND project funded by the European Commission; the program is offering funding opportunities for a 3-year post doc for a female researcher.

REWIRE has been designed to support researchers in their postdoctoral phase who intend to pursue an academic career. REWIRE has a focus on recruiting ambitious researchers who have the potential to embark on independent research careers.

The chemistry department of the university Vienna is equipped with 7 NMR spectrometers (from 400 to, 700 MHz, as well as a low-field spectrometer and access to DNP and EPR facilities), if you are interested in applying to the program or if you know anyone who might be interested to follow a career with a focus on NMR, please contact me (email: dennis.kurzbach@univie.ac.at; homepage: www.Vienna-DNP.at) for further information.

Kind regards,

Dennis Kurzbach

__________________________

Ass’t Prof. Dr. Dennis Kurzbach

University Vienna

Institute of Biological Chemistry

Währinger Str.38

1090 Vienna

Austria

Mail: dennis.kurzbach@univie.ac.at

Tel.: +43-1-4277-70523

www.Vienna-DNP.at

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[SharedEPR] Advanced EPR Summer School in Czech Republic, Nov 2019

Hello SharedEPR participants,

Here is an announcement from the European Federation of EPR groups (EFEPR):

EFEPR is organizing a summer school on Advanced EPR Spectroscopy in Brno in the Czech Republic, 18-25 November 2019. The website is here: http://eprschool.ceitec.cz

The school brings together about 100 graduate students / postdocs and more than a dozen outstanding EPR instructors – see the program here: http://eprschool.ceitec.cz/programme/. This is the 8th time this school is held, and it has been very successful in the past.

Consider going if you are a student; consider sending students if you are a PI. The school is very affordable: The total cost for this 8-day event is 420 EUR, or about 470 USD. This includes all lodging and food.

The registration deadline for the school is June 26, 2019 (in 9 days).

For questions, you can contact the local organizers via the school website: http://eprschool.ceitec.cz/contacts/

Best regards

Stefan Stoll

Research Fellow in Membrane Protein Structural Biology #EPR

Additional Information at: https://jobs.leeds.ac.uk/vacancy.aspx?ref=FBSBM1119

Are you an ambitious researcher looking for your next challenge? Do you have an established background in structural biology of membrane proteins? Are you interested in ion channels and mechanical sensing? Do you want to further your career in one of the UKs leading research intensive Universities?

Applications are invited to conduct research investigating the structure and function of mechanosensitive ion channels. These systems form pores in the cell membrane and allow the passage of molecules in response to membrane tension. 

A multi-disciplinary approach combining molecular/chemical (cloning, recombinant expression and purification), structural (CryoEM and PELDOR/DEER spectroscopy), functional (Electrophysiology) and computational (MD simulations) methods will be employed to investigate gating of these systems and characterise their unique states. CryoEM facilities in the Astbury Centre at University of Leeds are state-of-the-art, including 2 x Titan Krios 300keV electron microscopes. The project will also involve trips to the St Andrews and Manchester EPR facilities for PELDOR/DEER experiments.

We are seeking a highly motivated and talented individual interested in undertaking a challenging and exciting 3 year BBSRC-funded post in the laboratory of Dr Christos Pliotas. The project aims at obtaining and solving novel CryoEM structures of mechanosensitive ion channels in distinct conformational states. Dynamics will be interrogated by PELDOR/DEER and MD within lipid/native environment and function will be assessed by single molecule electrophysiology.

You should have a PhD (or close to completion) in Molecular/Structural Biology, Biochemistry, Biophysics or a closely allied discipline; with significant experience in expression, purification and structural biology of membrane proteins and ion channels.

To explore the post further or for any queries you may have, please contact: 

Dr Christos Pliotas, Lecturer in Integrative Membrane Biology

Tel: +44 (0)113 343 1229, email: C.Pliotas@leeds.ac.uk, University Profile, twitter: https://twitter.com/PliotasGroup

Location:Leeds – Main Campus

Faculty/Service: Faculty of Biological Sciences

School/Institute: School of Biomedical Sciences

Category: Research

Grade: Grade 7

Salary: £33,199 to £39,609 p.a.

Due to funding limitations it is unlikely an appointment will be made above £35,211 p.a.

Working Time: 100%

Post Type: Full Time

Contract Type: Fixed Term (3 years – external funding)

Release Date: Monday 03 June 2019

Closing Date: Sunday 14 July 2019

Reference: FBSBM1119

A spin-thermodynamic approach to characterize spin dynamics in TEMPO-based samples for dissolution DNP at 7 T field #DNPNMR

Jähnig, Fabian, Aaron Himmler, Grzegorz Kwiatkowski, Alexander Däpp, Andreas Hunkeler, Sebastian Kozerke, and Matthias Ernst. “A Spin-Thermodynamic Approach to Characterize Spin Dynamics in TEMPO-Based Samples for Dissolution DNP at 7 T Field.” Journal of Magnetic Resonance 303 (June 1, 2019): 91–104

 https://doi.org/10.1016/j.jmr.2019.04.012.

The spin dynamics of dissolution DNP samples consisting of 4.5 M [13C]urea in a mixture of (1/1)Vol glycerol/water using 4-Oxo-TEMPO as a radical was investigated. We analyzed the DNP dynamics as function of radical concentration at 7 T and 3.4 T static magnetic field as well as function of deuteration of the solvent matrix at the high field. The spin dynamics could be reproduced in all cases, at least qualitatively, by a thermodynamic model based on spin temperatures of the nuclear Zeeman baths and an electron non-Zeeman (dipolar) bath. We find, however, that at high field (7 T) and low radical concentrations (25 mM) the nuclear spins do not reach the same spin temperature indicating a weak coupling of the two baths. At higher radical concentrations, as well as for all radical concentrations at low field (3.4 T), the two nuclear Zeeman baths reach the same spin temperature within experimental errors. Additionally, the spin system was prepared with different initial conditions. For these cases, the thermodynamic model was able to predict the time evolution of the system well. While the DNP profiles do not give clear indications to a specific polarization transfer mechanism, at high field (7 T) increased coupling is seen. The EPR line shapes cannot clarify this in absence of ELDOR type experiments, nevertheless DNP profiles and dynamics under frequency-modulated microwave irradiation illustrate the expected increase in coupling between electrons with increasing radical concentration.

[NMR] PhD in computational spin dynamics

Peter Hore peter.hore@chem.ox.ac.uk via listes.univ-paris-diderot.fr 

PhD in computational spin dynamics at the Universities of Oxford and Oldenburg

We are seeking a graduate student with experience of, or an interest in, computational NMR or EPR who might be nervous of committing to a PhD in Brexit Britain but who would still welcome a strong connection to a leading UK university.

Based in the Department of Physics at the University of Oldenburg, Germany, the student will be supervised by Peter Hore (Department of Chemistry, University of Oxford). The position is funded by the DFG (https://www.sfb1372.de/) as part of Sonderforschungsbereich 1372: Magnetoreception and navigation in vertebrates: from biophysics to brain and behaviour. Further details can be found at: https://www.sfb1372.de/sig01-2.

For informal enquiries please email peter.hore@chem.ox.ac.uk.

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[NMR] Fwd: Technical/Scientific collaborator position in magnetic resonance at the University of Freiburg, Germany #EPR

De: Stefan Weber <Stefan.Weber@physchem.uni-freiburg.de>

Objet: Technical/Scientific collaborator position in magnetic resonance at the University of Freiburg, Germany

Date: 12 avril 2019 à 21:43:58 UTC+2

Dear colleagues,

Applications are invited for the post of technical/scientific collaborator to be held in the Institute of Physical Chemistry at the University of Freiburg, with effect from 1 July 2019. The appointment is full time and based on an indefinite term contract (salary according to TV-L E13). The successful candidate will be responsible for the technical administration and management of the magnetic resonance laboratories (NMR and EPR). S/he should have earned a university degree and hold a doctorate in either chemistry or physics. An excellent knowledge of magnetic resonance instrumentation for the development of home-built spectrometers and probe heads (in collaboration with the local electronics and mechanics workshops) and the operation and maintenance of the NMR and EPR instruments of the institute (including the lasers used for transient experiments), is required. The candidate is further expected to have vast experience with the measurement, analysis and interpretation of NMR and EPR spectra. Queries about the post and applications (including a letter of motivation, CV, and degree certificates with grades in a single PDF file), stating the job reference number 00000446, should be addressed to Prof. Dr. Stefan Weber, Albertstr. 21, 79104 Freiburg; E-mail: stefan.weber@physchem.uni-freiburg.de.

The closing date for applications is 24 April 2019. For further details, please see: http://www.uni-freiburg.de/verwaltung/stellenboerse/00000446 (only in German)

Kind regards,

Stefan Weber

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Electron Decoupling with Chirped Microwave Pulses for Rapid Signal Acquisition and Electron Saturation Recovery #DNPNMR

Barnes, Alexander, Nicholas Alaniva, Edward P. Saliba, Erika L. Sesti, and Patrick T. Judge. “Electron Decoupling with Chirped Microwave Pulses for Rapid Signal Acquisition and Electron Saturation Recovery.” Angewandte Chemie, March 28, 2019.

https://doi.org/10.1002/ange.201900139.

Dynamic nuclear polarization (DNP) increases NMR sensitivity by transferring polarization from electron to nuclear spins. Here we demonstrate that electron decoupling enables improved observation of DNP-enhanced 13C spins in direct dipolar contact with electron spins, thereby leading to an optimal delay between transients largely governed by relatively fast electron relaxation. Signal acquisition constitutes 12% of the total experimental time, significantly increasing signal-to-noise per unit time. We report the first measurement of electron longitudinal relaxation (T1e) during magicangle spinning (MAS) NMR through observation of DNP-enhanced NMR (T1e = 40 ± 6 ms, 40 mM trityl 4.0 kHz MAS, 4.3 K). With a 5 ms DNP period, electron decoupling results in a 195% increase in signal intensity. Chirped microwave pulses and MAS at 4.3 K are achieved with a custom spectrometer. MAS at 4.3 K, DNP, electron decoupling, and short recycle delays improves the sensitivity of 13C in the vicinity of the polarizing agent. This is the first demonstration of recovery times between MAS-NMR transients being governed by short electron T1 and fast DNP transfer.

Experimental quantification of electron spectral-diffusion under static DNP conditions #DNPNMR

Kundu, Krishnendu, Marie Ramirez Cohen, Akiva Feintuch, Daniella Goldfarb, and Shimon Vega. “Experimental Quantification of Electron Spectral-Diffusion under Static DNP Conditions.” Physical Chemistry Chemical Physics 21, no. 1 (2019): 478–89.

https://doi.org/10.1039/C8CP05930F.

Dynamic Nuclear Polarization (DNP) is an efficient technique for enhancing NMR signals by utilizing the large polarization of electron spins to polarize nuclei. The mechanistic details of the polarization transfer process involve the depolarization of the electrons resulting from microwave (MW) irradiation (saturation), as well as electron–electron cross-relaxation occurring during the DNP experiment. Recently, electron–electron double resonance (ELDOR) experiments have been performed under DNP conditions to map the depolarization profile along the EPR spectrum as a consequence of spectral diffusion. A phenomenological model referred to as the eSD model was developed earlier to describe the spectral diffusion process and thus reproduce the experimental results of electron depolarization. This model has recently been supported by quantum mechanical calculations on a small dipolar coupled electron spin system, experiencing dipolar interaction based cross-relaxation. In the present study, we performed a series of ELDOR measurements on a solid glassy solution of TEMPOL radicals in an effort to substantiate the eSD model and test its predictability in terms of electron depolarization profiles, in the steady-state and under non-equilibrium conditions. The crucial empirical parameter in this model is LeSD, which reflects the polarization exchange rate among the electron spins. Here, we explore further the physical basis of this parameter by analyzing the ELDOR spectra measured in the temperature range of 3–20 K and radical concentrations of 20–40 mM. Simulations using the eSD model were carried out to determine the dependence of LeSD on temperature and concentration. We found that for the samples studied, LeSD is temperature independent. It, however, increases with a power of B2.6 of the concentration of TEMPOL, which is proportional to the average electron–electron dipolar interaction strength in the sample.

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